Probe domestic switch needle KG300B head diameter 3.0 normally closed switch needle total length is 5

EL-C1600N100013-B

Test - lowercase jpg

Kaixin micro test

Key technical parameters of scientific research ELISA kit

The key technical parameters of the ELISA kit include intraplate variation, interplate variability, inter-assay variability, repeatability, specificity and cross-reactivity, and recovery. If the experiment is often carried out by ELISA, it is recommended to use the same manufacturer's kit, so as to ensure the repeatability of the data (of course, the consistency of the experimental conditions). For the quantitative ELISA kits for scientific research, the antibody pairs, enzymes and substrates, production processes and research and development strengths used by different manufacturers are not the same. Naturally, the data measured by different manufacturers' kits will be different.

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Select scientific research ELISA kit according to the experimental species and the nature of the sample to be tested

If the experimental sample of the experimental king is a human, mouse or rat sample, it is relatively easy to choose a suitable kit, but if your experimental sample is from other species such as chicken, cow, monkey, etc., it is available on the market. There are very few types of kits. It is not convinced that it can provide nearly 10,000 kits covering a wide range of suppliers. However, if it is necessary to test the indicators of these species, it is best to conduct a homology analysis of the test substance (if there is a species difference) and the species cross-reactivity data of the kit. For sample types, the general commercial kits are validated against serum, plasma, and cell culture supernatants. When selecting a kit, it is best to read the instructions in detail, such as whether the plasma sample anticoagulant is compatible with the kit. In addition, in some cases, there may be interference factors such as hemolyzed plasma, high fat content samples, human anti-mouse antibody (HAMA), and fetal bovine serum in the cell culture supernatant. Therefore, the kit should be selected according to the sample and, if necessary, appropriately optimized according to the sample. Or before sample collection, it is best to read the instructions for some reliable supplier kits or consult directly. If your sample is tissue or cell lysate, you need to be more careful in selecting the kit. Most commercial kits on the market have not been validated by tissue samples from multiple sources. This requires the experiment to understand that not all antibody pairs used in the kit can specifically detect your target protein from a pile of lysate proteins, but also consider the matrix effect of tissue samples. But if the experiment wants to understand the preparation of tissue samples, we will add another article to summarize.

Select a scientific ELISA kit based on the level of the analyte in the sample

If the experiment does not have any reference data for the level of the analyte in the sample, it is best to select a kit with a wide dynamic range. If the amount of the analyte in the sample is very low, it is recommended to select a kit with high sensitivity. In order to reduce the matrix effect, it is necessary to do at least 2-fold dilution with the diluent. If the content of the sample in the sample far exceeds the detection range of the kit (the range of the calibration), an appropriate multiple dilution is also required. When calculating the result at the end, multiply by the corresponding dilution factor.

Select scientific ELISA kit based on sample availability

In general, commercial kits use 50 ul or 100 ul of sample, but some suppliers have kits that require as little as 10 ul of sample. If your sample is precious or difficult to obtain, you need to carefully consider the kit's sample usage requirements. Care should be taken if the sample size is small and multi-factor detection is required.