The tester detailed tricks! How to diagnose and repair high performance liquid chromatography instrument - Database & Sql Blog Articles

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High performance liquid chromatography instrument

Faults can be started from many aspects: First, it is estimated from the operation of the components, such as changes in pressure and flow rate. The second is to estimate from the abnormal situation of the chromatogram, such as peak time and peak shape. The third is to analyze from the data results. Below, Xiaobian will detail the diagnosis and maintenance of high performance liquid chromatography instrument faults.

I. Judging from pressure changes

No pressure

2. High pressure

3. Low pressure

4. Unsteady pressure

Second, speculate from the abnormal situation of the chromatogram

Peak front

2. Peak tailing

3. Peak broadening

4. Peak bifurcation

5. No peak

6. Negative peak

7. Ghost Peak

8. Retention time changes

9. Retention time keeps changing

10. Baseline drift

11. Irregular baseline noise

12. Regular baseline noise

Third, analyze from the data results

Quantitative result

2. Qualitative results

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